Guiding Stem Cell Differentiation and Proliferation Activities Based on Nanometer-Thick Functionalized Poly-p-xylylene Coatings

Modifications of biomaterials based on the combination physical, chemical, and biological cues for manipulating stem cell growth are needed modern regenerative medicine. The exploitation these sophisticated modifications remains a challenge, including substrate limitation, biocompatibility, versatile general activities. In this report, vapor-phase coating technique functionalization poly-p-xylylene (PPX) was used to generate surface modification use with cells in culture. provided ability covalent conjugation that immobilized bone morphogenetic protein 2 (BMP-2) fibroblast factor (FGF-2), modified surfaces enabled direct differentiation controlled proliferation because specific ligations were realized between factors maleimide-modified surface, reactions proceeded high specificity rapid kinetics under mild conditions. densities approximately 140 ng·cm−2 BMP-2 155 FGF-2. Guiding activities human adipose-derived (hADSCs) achieved by modifying promote hADSC capacity rate. reported system demonstrated substrate-independent conformity, stability, it could provide an effective interface platform further biomedical applications.